Media Center
Talk about hope in propolis.
2nd WPSF Treatise (Antioxidative activities of non-alcoholic water-soluble propolis (WEEP) in mouse
PROBEE
2016-09-23
Number of views 6,947
Antioxidative activities of non-alcoholic water-soluble propolis (WEEP) in mouse and human
Sung-Kee Jo1*, Young-Soo Jin2, Uhee Jung1, Seung-Wan Lee3, Yong-Kap Hur3
1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute Jeonbuk 580-185, Korea, 2Asan Medical Center, Seoul 138-736, Korea 3Seoul Propolis, Co. Ltd. Daejeon 305-353, Korea
In this study, we have investigated the antioxidative activities of non-alcoholic water-soluble propolis (WEEP) in vitro, in mouse, and in human. In vitro studies showed that WEEP had hydroxyl radical and DPPH radical scavenging activities comparable to those of ethanol extracted propolis (EEP). In mouse studies, the oral administration of WEEP significantly decreased the CCl4-induced malondiadehyde level in the liver and serum (70% inhibition). WEEP also increased the survival of bone marrow stem cells and decreased DNA damage (micronucleus) in γ-irradiated mice, indicating that WEEP effectively reduced the radiation-induced oxidative damage. In these mouse experiments, WEEP showed antioxidant activities comparable to those of EEP. We next investigated the antioxidative effects against exercise-induced oxidative stress in humans. Forty untrained people were supplemented with placebo or WEEP (990 mg/day) for 2 weeks, and exercise-induced oxidative stress was imposed by treadmill exercise at the 110% AT level. The effects of WEEP supplementation on the blood antioxidant and inflammation biomarkers (MDA, TAS, IL-6, TNF-α) were analyzed. In both groups, all biomarkers did not show statistically significant changes after the supplementation. However, there were trends that the group supplemented with WEEP showed decreased MDA and increased TAS after exercise, which implied the antioxidant effects of WEEP in humans. On the other hand, the factors associated with immune and inflammatory responses (IL-6 and TNF-α) were not altered by WEEP supplementation in this moderate exercise load model. In conclusion, our results showed that WEEP can exert antioxidative effects in mice and humans, which provided a good rationale for its use for prevention of various diseases associated with oxidative stress.
Keywords: propolis, WEEP, antioxidant, exercise
2009-08-07 14:19:53 , Friday